Fig. 1 | Nature Communications

Fig. 1

From: Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants

Fig. 1The alternative text for this image may have been generated using AI.

Library-based screen for the effects of histone variants and PTMs on de novo H2B ubiquitylation. a Cartoon illustrating how pre-existing epigenetic marks (represented by different colored flags and quadrants) on the chromatin template might modulate deposition of the H2BK120ub mark. b Schematic of the experimental workflow used in the in vitro screen. A DNA-barcoded mononucleosome library containing a wide range of histone and DNA modifications is exposed to HA-tagged ubiquitin and the requisite ubiquitylation apparatus corresponding to the purified recombinant E1, E2, and E3 ligases. De novo ubiquitylated products are then enriched using the HA tag and analyzed by deep sequencing. For clarity, only one modification per nucleosome is shown

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