Fig. 6 | Nature Communications

Fig. 6

From: HOXA9 inhibits HIF-1α-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development

Fig. 6

HOXA9 represses glycolytic genes by association with CRIP2. a The chromatographic analysis of the protein mixes immunoprecipitated by HOXA9 antibody or control IgG. Arrows indicate the identified CRIP2 peptide peaks in the HOXA9-pulldown sample, which were lacking in control IgG sample. b CRIP2 was verified to be the interacting partner of HOXA9 by Co-IP followed with western blot detection. IP immunoprecipitation, WB western blot. c Co-IP of both HOXA9 and CRIP2 using specific antibodies was performed and followed with western blot detection to confirm their direct interactions. Each experiment was performed at least in triplicate

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