Fig. 7 | Nature Communications

Fig. 7

From: HOXA9 inhibits HIF-1α-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development

Fig. 7

The glycolysis-inhibitory role of HOXA9 is dependent on CRIP2. a Western blot detection showed that CRIP2 knockdown led to significant upregulation of HIF-1α, HK2, GLUT1, and PDK1, whereas HOXA9 expression was not affected. b–d Depletion of CRIP2 enhanced cell proliferation, migration, and invasiveness of A431 cells by CCK-8 assay, transwell migration assay, and Matrigel invasiveness measurement. e, f ECAR and OCR analysis of A431 cells following depletion of CRIP2 by siRNA as summarized from raw data. g Western blot detection showing the variations of protein expression levels of HOXA9, CRIP2, HIF-1α, HK2, GLUT1, and PDK1 in response to CRIP2 knockdown and/or HOXA9 overexpression. h, i ECAR and OCR analysis of A431 cells depleted of CRIP2 by siRNA and/or overexpressing HOXA9 as summarized from raw data. Each experiment was performed in triplicate and data are presented as mean ± s.d. One-Way ANOVA and Dunnett’s multiple comparison test were used to analyze the data (*P < 0.05, **P < 0.01, ***P < 0.001)

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