Fig. 2
Deletion of the 20q-TERRA locus decreases telomere length. a Representative frequency graphs of telomere length distribution (a.u.) measured in the U2OS WT pool (black), in the WT clones (#1 and 2; dark gray), and in the 20q-TERRA KO clones (#A7, E1, E6, and C4; light gray). The mean telomere length and the number of telomeres analyzed is shown. The red lines are arbitrary lines placed in the exact same position in each frequency graph to visualize differences between samples. b Graph showing the quantification of the mean telomere length in the U2OS cells WT pool, the WT expanded clones (#1 and 2), and in the 20q-TERRA KO clones (#A7, E1, E6, and C4) by HT-Q-FISH (mean values ± s.e.m., n = technical replicates). c Graph showing the percentage of short telomeres in the same settings. Short telomeres are considered those in the 10% percentile of the total telomere length distribution (mean values ± s.e.m., n = technical replicates). One-way ANOVA with Dunnett’s post test was used for the statistical analysis (*p < 0.05, **p < 0.01, and ***p < 0.001)
