Fig. 1
From: Cathelicidins prime platelets to mediate arterial thrombosis and tissue inflammation
Cathelicidins are present in human and mouse arterial thrombi. a, b Representative images of coronary artery thrombi isolated from five patients with acute myocardial infarction. a Immunohistochemistry for LL-37 indicated enrichment within leukocytes (arrowhead), but also stained leukocyte-free areas (asterisk). Bars, 200 µm (overview) and 10 µm (magnification). b Immunofluorescence analysis of LL-37 (red), myeloperoxidase (MPO, yellow), CD41 (platelets, green), and DAPI (nuclei, blue). Bar, 10 µm. c, d Representative images of murine carotid artery thrombi generated by ferric chloride injury. c Immunohistochemistry for cathelicidin-related antimicrobial peptide (CRAMP, mouse homologue for LL-37) indicated enrichment within leukocytes (arrowhead), but also stained leukocyte-free areas (asterisk). Bars, 10 µm. d Immunofluorescence analysis of CRAMP (red), CD41 (platelets, green), and DAPI (nuclei, blue). Bar, 10 µm. e Analysis of CRAMP binding in arterial thrombosis in vivo. 5-FAM-labeled CRAMP or scrambled control was injected into wild type mice before induction of ferric chloride injury. Platelets were labeled in vivo using a DyLight649-labeled non-blocking GPIbβ antibody. Left: 5-FAM-labeled CRAMP (green) associated with platelets (GPIb, red in merged image) in the forming thrombus. Right: Image for 5-FAM-labeled control peptide and platelets (GPIb, red in merged image). Bar, 500 µm. See also Supplemental Movies 1, 2. f Flow cytometry analysis of LL-37 binding to isolated human, platelets in vitro. 5-FAM-labeled LL-37 (red), scrambled 5-FAM-labeled control peptide (blue), or vehicle (gray). Graph shows mean and SEM. P-value was determined by unpaired t-test
