Fig. 6

CRAMP-activated platelets promote neutrophil recruitment and extravasation. a–f Intravital microscopy of the mouse cremaster muscle following trauma-induced inflammation. Platelets were isolated and pretreated ex vivo with CRAMP or vehicle. 10⁷ platelets were then injected into lysozyme 2 (LysM)-eGFP (neutrophil reporter) recipient mice. a Tethering of CRAMP-pretreated platelets in cremaster muscle venules and arterioles (n = 8 vessels each, see also Supplemental Movies 3, 4). b Representative intravital epifluorescence microscopy images of neutrophils (white). Bar, 20 µm. c Platelet–neutrophil aggregates formation in whole blood of GFP-positive neutrophils and transfused platelets that were pretreated with CRAMP or vehicle control (n = 3). d Neutrophil rolling, e adhesion efficiency, and f extravasation in cremaster muscle venules (n = 10–12 vessels of four different animals). Graphs show mean and SEM. P-values were determined by unpaired t-test