Fig. 3 | Nature Communications

Fig. 3

From: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers

Fig. 3

Difference in astrocyte-synapse structural interactions in layers II/III and VI. a Series of SBF-SEM images acquired from coronal sections of the brain of an adult wild-type mouse at ×4730 magnification. b Procedure for quantification of astrocyte ensheathment of synapses. Serial SEM images were reconstructed to measure the perimeter of synaptic axon-dendrite interface and the astrocytic perimeter. c–e Quantification of astrocyte ensheathment of synapses in bin2 and bin4 (n = 16 synapses in each bin for control (c) and n = 15 synapses in each bin for reeler (d) and Dab1 cKO (e) mice). Astrocyte position in the cortical layers is expressed as relative distance from the corpus callosum (CC) to the pia: bin2, 0.65–0.9; and bin4, 0–0.25. Horizontal bars represent mean values. ***P < 0.001 (Welch’s t-test)

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