Fig. 7
Disruption of layer-specific astrocyte orientation in reeler and Dab1 cKO mice. a Immunofluorescence staining for S100β in bin2 (layer II/III) and bin4 (layer VI) of coronal brain sections from control (Dab1fl/fl), Dab1 cKO (Dab1fl/fl;NexCre/+), and reeler (Reln−/−) mice at P60–P70. Arrowheads indicate S100β-positive astrocytes. Scale bars, 50 µm. b Schematic for measurement of astrocyte orientation relative to the brain surface based on S100β immunostaining. Scale bar, 10 µm. c Quantification of the orientation angle of astrocytes (S100β-positive, Sox10-negative cells) in bin2 and bin4 of control, Dab1 cKO, and reeler mice (n = 3 mice) at P60–P70. Horizontal bars indicate median values. Control, n = 106 and 68 cells; Dab1 cKO, n = 100 and 76 cells; reeler, n = 96 and 74 cells for bin2 and bin4, respectively. ***P < 0.001 (Welch’s t-test)
