Fig. 1
From: RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex
Mapping the interactions in human R2TP core components. a A cartoon for sequence and domains of the components of the human R2TP complex. b GST pull-down experiments depicting the interactions between the several regions in RPAP3 and PIH1D1. FL stands for full length, CS for the CS domain in PIH1D1, and MW for molecular weight markers. Be aware that for simplification, several PIH1D1 and RPAP3 constructs are indicated within the same lines on top of the gel. Some minor contaminants are present in some of the samples. c Pull-down experiments showing that removal of residues 401–420 from an RPAP3 construct eliminates the interaction with the CS domain in PIH1D1. d Pull-down experiments demonstrating the interaction of RPAP3–RBD with RUVBL2. This interaction is not affected when the DII domains in RUVBL2 are removed
