Fig. 6
Kinesin-2 accumulates at the base of deglutamylated cilia. a Neon-Kif3B-stable NIH3T3 cells were transfected with CCP5CD–Cerulean3–FKBP–P2A–mCherry–FRB–MAP4m. Transfected cells at 80–90% confluency were serum-starved for 24 h and then treated with 100 nM rapamycin for 30 min. Scale bar, 4 μm. b Linescan profile of Neon-Kif3B from the base to the tip of the cilium in a. c Neon-Kif3B-stable NIH3T3 cells were transfected with P2A-based constructs for co-expression of mCherry–FRB–MAP4m and Cerulean3–FKBP-tagged proteins. Transfected cells at 80–90% confluency were serum-starved for 24 h and then treated with or without 100 nM rapamycin for 30 min. The percentage of Neon-Kif3B at the base (Base) or axoneme region (Axo) of control or deglutamylated cilia is plotted. Data represent the mean ± s.e.m. (n = 74, 48, and 69 cells for the Cerulean3–FKBP, CCP5CD–Cerulean3–FKBP, and CCP5CDDM–Cerulean3–FKBP groups, respectively; three independent experiments). NS and * indicate no significant difference and P < 0.05, respectively, between the conditions in the presence and absence of rapamycin (Student's t-test)
