Fig. 2
GATA2 regulation by matrix stiffness in LECs. a, b qRT-PCR analysis of GATA2 in human LECs (a) and primary mouse LECs (b) grown on soft (0.2 kPa) or stiff (25 kPa) matrix. n = 3 experiments, mean ± s.e.m. p value, one-sample t-test. c Immunofluorescence of human LECs grown on stiff and soft matrix using antibodies against GATA2 (green) and VE-cadherin (red), and for DAPI to show nuclei (grey). LECs grown on soft matrix exhibit an overall higher expression of GATA2 as indicated by higher immunofluorescence intensity in both the nucleus and cytoplasm and have an elongated shape and a distorted nucleus. d Quantification of nuclear and cytoplasmic GATA2 protein in human LECs grown on soft (0.2 kPa) or stiff (25 kPa) matrix. Data represent mean pixel intensity (n = 8 images with 8–24 cells per image (soft), and n = 8 images with 21–37 cells per image (stiff) from 3 experiments) ± s.e.m. p value, unpaired Student’s t-test. Scale bars: 50 µm
