Fig. 1
Snail expression is related to epithelial-to-mesenchymal transition and poor prognosis in ovarian cancer. a Correlation between generic epithelial-to-mesenchymal transition (EMT) scores for 266 high-grade serous ovarian cancer (HGSOC) samples from TCGA dataset1 and Snail expression. The EMT signature was acquired from a previous report by Tan et al.3. Generic EMT scores were calculated following the method of single-sample Gene Set Enrichment Analysis (ssGSEA). The correlation coefficient (R) and P-value were based on Pearson’s product-moment correlation analysis; R = 0.41 P < 0.0001. b Snail expression in HGSOC samples representing four subtypes from the TCGA datasets; ***P < 0.001 and ****P < 0.0001, mesenchymal vs. the other three subtypes based on one-way ANOVA with Tukey’s multiple comparisons test. Bars: mean and SEM. c Classification of HGSOC as determined by the intensity of nuclear Snail immunostaining. Cases with scores of 0/1 were assigned to the Snail-low group, whereas those with scores of 2/3 were assigned to the Snail-high group to be used in survival analysis. Scale bars, 100 μm. d Overall survival curves for patients with HGSOC in Kyoto University Hospital (n = 56; 48 stage III cases and 8 stage IV cases), based on Snail staining of disseminated tumors in the omentum. P = 0.021 by log-rank test
