Fig. 3

Snail is associated with increased intratumoral myeloid-derived suppressor cells. a Immunostained cell count in HM-1-control and HM-1-shSnail (sh1) subcutaneous tumors from immunocompetent mice. HPF high power field; n = 5–6. CD8⁺ (left), Gr-1⁺ (middle), and CD11b⁺ (right). b Flow cytometry of HM-1-control and HM-1-shSnail (sh1) subcutaneous tumors from immunocompetent mice. The percentage of positive cells is plotted; n = 6. CD8⁺ (left; CD3⁺CD4⁻CD8⁺), intracellular IFNγ (middle; IFNγ CD3⁺CD8⁺), and MDSC (right; CD45⁺Gr-1⁺CD11b⁺). c Representative image showing CD33 expression (a marker of human MDSC) in peritoneal dissemination of human high-grade serous ovarian cancer (HGSOC). Scale bar, 100 μm. d Correlation between infiltration of CD33⁺ cells and Snail expression in corresponding disseminated tumors in the omentum (R = 0.35, P = 0.0082) of HGSOC; n = 56; Pearson’s product-moment correlation analysis. *P < 0.05, **P < 0.01, and ***P < 0.001 (unpaired t-test in a and b). Averaged data are presented as the mean ± SEM