Fig. 6
PI3Kγ controls the heart parasitism after infection with T. cruzi. a Representative images from H&E staining of heart tissue from WT (n = 6) and Pik3cg−/− (n = 6) mice 18 days post infection with T. cruzi Y strain. Black arrows indicate amastigote nests of the parasite. Scale bars = 20 µm. b, c Quantitative PCR analysis of T. cruzi DNA isolated from Pik3cg−/− (b) and Pik3cgKD/KD (c) mice 18 days post infection with T. cruzi Y strain. d Survival rate of Pik3cg−/− mice infected with 103 trypomastigote forms of T. cruzi Y strain and treated with 100 mg Kg− of benznidazole (BNZ) (n = 8) or vehicle (n = 6). *P < 0.05, by unpaired Student’s t-test, relative to WT mice (a–c). *P < 0.05 and ***P < 0.001 (unpaired Student’s t-test in a–c and Mantel–Cox log-rank test in d). Data are from one experiment (d) or representative of two (c) or three (a, b) independent experiments (mean ± s.e.m in a–c)
