Fig. 6

Oxidative inhibition of GAPDH is transient and acute oxidative stress reduces mitochondrial respiration. Metabolite abundance of GAPDH substrate (a), downstream metabolite (b), and resultant relative GAPDH enzymatic activity (c) after treatment with 500 µM hydrogen peroxide for 15 min and subsequent cellular recovery. d Relative oxygen consumption rate (OCR) in response to treatment with 350 µM hydrogen peroxide. Arrow indicates addition of hydrogen peroxide. a–c Mean (S.D.) of three independent experiments with triplicate measurements; d mean (S.D.) of three independent experiments with 12 replicate measurements per treatment. **p value = 0.0052 (two-way ANOVA with Dunnet’s test for multiple comparisons correction)