Fig. 8

Analysis of mouse kidneys shows oxidation of proteins from various tissue compartments. a Representative image of Fh1^fl/fl and Fh1^−/− mouse kidney tissue slices stained for H&E, taken at 20× magnification. Scale bar indicates 100 µm. b Density scatterplot displaying log 2 median peptide oxidation ratios vs. peptide intensity in Fh1^−/− mouse kidney tissue compared to Fh1^fl/fl kidney tissue. Every square represents a unique peptide; colour scale of the density of the data points in the corresponding region is indicated on the right. Highlighted green circles are significantly oxidised (positive values) and reduced (negative values) peptides. Gene names of corresponding peptides are displayed for the most significantly oxidised or reduced peptides. c Venn diagram showing overlap and exclusivity of proteins involved in cell redox homoeostasis as defined by GOBP that were significantly modified in the acute and chronic oxidative stress cell and kidney models. d Comparison of components of the mitochondrial electron transport chain complexes that were significantly modified in the acute and chronic oxidative stress cell and kidney models. a Representative images of single histology slides; b based on the comparison of 1 mouse per genotype, using four replicate tissue slices per mouse. c, d Based on four independent experiments, single measurement (H₂O₂ model, Fh1 cell model) or the comparison of one mouse per genotype, using four replicate tissue slices per mouse (Fh1 tissue model)