Fig. 6
From: Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence
Dormant cells activate a BECN1-independent autophagy pathway. a Up and downregulation of breast cancer dormancy-related signalling pathways in D2.0 R cells on BME after 5 days in culture as compared with D2.0 R cells on BME after 1 day in culture. b Dot plots representing the differential expression of selected autophagy genes in D2.0 R cells on BME matrices as compared to D2.0 R cells on BME plus COL1 matrices at day 5 of culture (n = 3 independent samples per condition. Comparisons by Partek Gene Specific Analysis (GSA) algorithm, false discovery rate (FDR) set at 0.05. *P = 5.45 × 10−5; **P = 4.25 × 10−6; ***P = 3.05 × 10−4; ****P = 9.31 × 10−6). c Graphical summary of the expression profile of canonical autophagy genes in D2.0 R cells on BME after 5 days in culture as compared with D2.0 R cells on BME plus COL1 after 5 days in culture. d Representative western blot of autophagy markers from D2.0 R cells plated on BME or BME plus COL1 with or without HCQ on days 1 and 5. e Total lung surface metastatic burden in CD1nu/nu mice injected with D2.0R-GFP stably expressing shSCR (scrambled), shBECN1 (shRNA 1 and 3) (upper panel) or shATG7 (shRNA 4 and 5) (lower panel)
