Fig. 1
From: Activity dependent LoNA regulates translation by coordinating rRNA transcription and methylation
Identification of nucleolar lncRNAs from trained mouse brain and activated neurons. a rRNA levels (45S, 28S, 18S, and 5.8S) in hippocampal brain from spatial learning-induced mice in Morris water maze and control mice (24 h after training, n = 5 for each group), as measured by qPCR. Data were normalized against U1 snRNA. b, c Total RNA was extracted from N2a cell nucleoli, and fragments longer than 200 nt were subjected to RNA-seq. b Relative abundances of mRNA and lncRNA in nucleoli. c Distribution of detected nucleolar lncRNA RNA-seq peaks. d qPCR validation of expression for the 30 most abundant lncRNA in the nucleoli of N2a cells (n = 3), expression is presented as fold difference over Gm15758 (the least abundant one among the top thirty). e Expression of the 30 most abundant lncRNAs (validated in d) in hippocampal brain of experience induced and control mice (n = 5), as determined by qPCR. f Expression of the ten most significantly downregulated lncRNAs (determined in e) in KCL-activated primary neurons at various time point (n = 3 at each time point), determined by qPCR. In a, d, e, f, error bars, s.e.m.; *P < 0.05; **P < 0.01; ***P < 0.001 by ANOVA or two-tailed Student’s t test
