Fig. 6 | Nature Communications

Fig. 6

From: An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins

Fig. 6

The synergistic interactions dramatically enhanced the enrichment of O-GlcNAcylated peptides in human cells. a Comparison of glycoproteins with one HexNAc identified with BA and DBA, which clearly shows that the results from DBA are substantially better. b Distribution of O-glycoproteins modified with HexNAc(1) identified in HEK 293T cells based on the cellular compartment. c Proposed mechanism of the interactions between DBA and GlcNAc benefiting from synergistic interactions. d Cellular compartment distributions of glycoproteins containing one HexNAc identified in the three types of human cells (light blue—MCF7, dark blue—Jurkat, and green—HEK 293T)

Back to article page