Fig. 2

Mousepox pathogenesis in the absence of ECTV CrmD. Groups of 10 (a, c, e, g) or 5 (b, d, f, h) BALB/c mice were inoculated s.c. in the left hind footpad with 10 PFU or 1000 PFU, respectively, of ECTV (white circles), ECTVΔCrmD (black triangles), ECTVRevCrmD (black circles), ECTVRevCRD (white triangles) or PBS alone (black squares), and monitored daily for weight loss (a, b), signs of illness (c, d) and footpad swelling (e, f), as indicated. Data are shown as mean +/− SEM. Statistical analyses were performed using multiple t-tests with false discovery rate at Q = 1%. Analyses were performed up to times post infection at which survival rates in the corresponding group were above 50%. Black bars indicate time points at which significant differences (p < 0.01) between ECTV− / ECTVRevCrmD− and ECTVΔCrmD-inoculated mice were found. Asterisks indicate time points at which significant differences (p < 0.01) between ECTVΔCrmD and ECTVRevCRD-inoculated mice were found. g, h The number of surviving animals during the course of infection. Data are from one experiment representative of three independent experiments