Fig. 8
From: Chemokines cooperate with TNF to provide protective anti-viral immunity and to enhance inflammation
Inhibition of TNF activity in vivo impairs NK cell activation in response to ECTV infection. Cells from DPLN (a–d) harvested at 2 dpi or from spleens (a, e, f) collected at 7 dpi from PBS-inoculated BALB/c mice or mice infected with 105 pfu of the indicated viruses were analyzed by flow cytometry using conjugated anti-CD3, anti-CD8, anti-DX5 and anti-granzyme B (GzB) antibodies. In a, representative dot plots of the staining of NK cells (CD3– DX5+; top panel) and CD8 T cells (CD3+CD8+; bottom panel) isolated from DPLN or spleen, respectively, are shown. Number inside each graph indicates the % of cells inside the depicted gates. In b and c, a quantification of the total number and % of NK cells, respectively, is presented. The % of NK cells expressing granzyme B in each group is quantified in d. In e and f, a quantification of the total number of CD8 T cells and granzyme B-expressing CD8 T cells detected for each group is shown. The number of events positively stained with the corresponding isotype control antibodies (isotype DX5, 0.15% positives; isotype CD8, 1.3% positives; isotype granzyme B, 0.02% and 0.03% positives in DPLN and spleen, respectively) were subtracted from each sample for the quantification analyses. Data are mean +/− SD from one experiment representative of three independent experiments with 4–5 animals per group. Statistically significant groups are indicated (asterisks p < 0.05, ANOVA with Bonferroni multiple comparison test)
