Fig. 2
From: The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation
Conjugation of ebselen and ebsulphur to SOD1 variants increases the binding affinity of the dimer. a SOD1 variants (wild-type, A4V, H46R/H48Q, G93A and I149T) with ebselen (red), ebsulphur (blue) and without treatment (black) were buffer exchanged into 200 mM NH4OAc and subjected to nanoESI mass spectrometry at different protein concentrations under gentle conditions optimized to maintain non-covalent interactions. Curves show the percentage of monomer signal as a function of protein concentration with determined dimer Kd values inset for each SOD1 variant. Error bars represent the standard deviation of three replicates. b The fold-increase in binding affinity of each SOD1 variant when ebselen or ebsulphur is conjugated at Cys111 where error bars represent the upper and lower Kd values calculated in a
