Fig. 3
From: TAK1 activation of alpha-TAT1 and microtubule hyperacetylation control AKT signaling and cell growth
Structural determinants of TAK1 required for αTAT1 binding. a Schematic representation of TAK1 WT and C-terminal truncated mutant TAK1-ΔCT. b COS-7 cells expressing HA-αTAT1 WT, Flag-TAK1, or Flag- TAK1-ΔCT were immunoprecipitated with Flag antibody. c COS-7 cells expressing HA-αTAT1 WT, Flag-TAK1, or Flag-TAK1-ΔCT were immunoprecipitated with αHA antibody. d COS-7 cells expressing Flag-TAK1 WT, Flag-TAK1 KD (K63W), and Flag-TAK1-ΔCT were fixed and imaged for Flag (left panel) and Acetyl-tubulin (right panel) immunofluorescence co-staining. CTCF was quantified using Image J, at least 30 cells were quantified per condition. The images are representative of three independent experiments, n = 3. Error bars represent SEM and type 2 t-test analysis show relative to control: *not significant; **p < 0.001. e Schematic representation of αTAT1-WT and truncation mutants αTAT1-Δ301, αTAT1-Δ196. f COS-7 cells expressing Flag-TAK1 WT, HA-αTAT1 WT, HA-αTAT1-Δ196, or HA-αTAT1-Δ301 were immunoprecipitated with α-HA antibody. g, h COS-7 cells expressing HA-αTAT1 WT, HA-αTAT1-Δ196, or HA-αTAT1-Δ301 were fixed and imaged for α-HA (left panel) and Acetyl-tubulin (right panel) immunofluorescence co-staining. CTCF was quantified using Image J, at least 30 cells were quantified per condition. The images are representative of three independent experiments, n = 3. Error bars represent SEM and type 2 t-test analysis show relative to control: *not significant; **p < 0.001
