Fig. 4
LbCpf1 targeted to Vegfa or Hif1a does not affect cone function. a–c At day 42 post-AAV injection, full-field ERG was performed to evaluate retinal function. There was no significant decrease in the a scotopic a wave, b scotopic b wave, or c photopic response in mice treated with AAV-Cpf1-Vegfa or -Hif1a compared to normal control mice. Error bars indicate s.e.m. (n = 4). One-way ANOVA. ns, not significant. d, e Opsin-positive areas in the retina at day 42 post-injection. d Relative opsin-positive areas of the AAV-Cpf1-injected mice were normalized to that of the AAV-uninjected negative control mice. Error bars indicate s.e.m. (n = 4). Student’s t-test. ns not significant. e Representative images of opsin positive areas in mice treated with AAV-Cpf1-Vegfa or -Hif1a compared to the AAV-uninjected negative control mice (which received no AAV). Scale bar 20 μm
