Fig. 1

Intrapancreatic duct pressure causes acute pancreatitis. a Test solutions were infused into the pancreatic duct of mice as illustrated. Injection pressures were continuously monitored using a pressure transducer. b Injection of 400 µL buffered saline containing methylene blue dye was distributed throughout the pancreas. The white line indicates the boundary of the pancreas and yellow line indicates the mid-region of the pancreas that was used for histology and myeloperoxidase (MPO) determinations. Either sham-operated animals (control) or buffered saline solutions were infused at 25–33 mm Hg (pressure) and c edema, d blood amylase, e MPO, and f histological scoring were analyzed 24 h after duct injection in C57BL/6J male mice. Statistical analysis was performed using Student’s t test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 (n = 7–13 for control and 13–17 for pressure). g Representative images of pancreatic tissue stained with H&E. Bar = 100 µm