Fig. 4
From: Piezo1 is a mechanically activated ion channel and mediates pressure induced pancreatitis
Genetic deletion of Piezo1 in pancreatic acinar cells reduces the severity of pancreatitis. a Confocal microscopic images of pancreatic sections from littermate wild-type (left panel) and Piezo1aci KO (right panel) mice imunostained for Piezo1 (red) and trypsin (green). DAPI-stained nuclei are blue. ×63 objective. Bar = 10 µm. Buffered saline was injected into the pancreatic duct at 25–33 mm Hg (pressure) of Piezoaci KO or littermate wild-type (WT) mice. Control animals were subjected to sham operation. Tissues were harvested 24 h post injection and analyzed for b pancreatic edema, c blood amylase, d MPO, and e histological scoring. The number of animals in each group is indicated in the respective column. Statistical analysis was performed using ANOVA test with Tukey’s post-test. *P ≤ 0.05; ***P ≤ 0.001. f Representative images of pancreatic tissue stained with H&E taken with a ×10 objective. Bar = 100 µm
