Table 1 High-content imaging and analysis of Plasmodium liver stage (LS) parasites

From: A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum

Parasite population

Size category

Area

Mean intensitya

Maximum intensitya

Cell roundness

Small forms:

 

1

>15 μm2

<100 μm2

>1500

>3000

>0.80

Large forms:

 A

2

>99 μm2

<300 μm2

>800

>1500

>0.70

 B

3

>299 μm2

<500 μm2

>250

>1000

>0.70

 C

4

>499 μm2

<1000 μm2

>250

>1000

>0.70

 D

5

>999 μm2

<2500 μm2

>200

>300

>0.60

 E

6

>2499 μm2

>200

>600

>0.38

  1. Defined fluorescent imaging parameters and thresholds used to identify and quantify Plasmodium LS parasites using Perkin Elmer Harmony software. Small forms refer to P. vivax hypnozoites (mean ~30 µm2) but with wide size range also represents for P. falciparum day 6 schizonts (mean ~80 µm2). Large forms refer to developing schizonts and are further categorized into 5 distinct groups based upon area and intensity
  2. a Based on FITC channel