Fig. 3
From: Structural basis of actin monomer re-charging by cyclase-associated protein
Structural mechanisms of ADP-G-actin and ATP-G-actin interactions with CAP1. a The domain architecture of CAP1. Oligomerization domain (OD), helical folded domain (HFD), polyproline region 1 (PP1), WH2 domain, polyproline region 2 (PP2), and CARP domain. The CAP1 regions in co-crystal structure and atomistic MD simulations model are indicated in cyan and in yellow, respectively. b MD simulation model of the ATP-actin–WH2 domain complex (System 3, Supplementary Table 3). The regions important for ATP-actin binding as determined by mutagenesis are highlighted in orange and red. Actin subdomains 1–4 are indicated by circles. c The ADP-G-actin–CAP1248–474 complex model (System 2, Supplementary Table 3) from MD simulations. The regions important for ADP-G-actin binding are indicated in orange and red, and the C-terminal tail of CAP1 is in magenta. For detailed effects of the mutants on actin binding, see Table 1 and Supplementary Fig. 4
