Table 1 Biochemical analysis of CAP1 mutants

			G-actin binding		Nucleotide exchange
	Mutant #		ATP-actin	ADP-actin
		Wild type	++	++++	++++
WH2 domain	1	R253A L256A I260A	−	+++	++
	2	Δ²⁶⁶ITHA²⁶⁹	−	+++	+
	3	²⁷⁰LKHV²⁷³ → ²⁷⁰AAAA²⁷³	−	+++	−
	4	²⁷⁹THKN²⁸² → ²⁷⁹AAAA²⁸²	+	++++	++++
CARP domain primary interface	5	K347A Y351A Y353A	++	+	+++++
CARP domain primary interface	6	K365A N367A D372A	++	−	−
CARP domain secondary interface	7	D446A E449A	++	+	+++++
	8	Δ⁴⁷¹EIAG⁴⁷⁴	++	+++++	++
	9	Y418A F447A	++	−	−
	10	L339A Q399A D446A	++	++	+++++

A summary table of C-CAP mutant affinities for ADP-G-actin and ATP-G-actin, and their effects on nucleotide exchange on ADP-actin monomers (see Supplementary Figs. 3c, d, 4). Symbols: K_d for ATP-actin: ++ (0.5–2 μM), + (2–5 μM), – (unmeasurable). K_d for ADP-actin: +++++ (0.005–0.025 μM), ++++ (0.025–0.1 μM), +++ (0.1–0.3 μM), ++ (0.3–1 μM), + (>1 μM), – (unmeasurable). Half-times of nucleotide exchange: +++++ (2–8s), ++++ (8–15s), +++ (15–19s), ++ (19–24s), + (24–28s), – (>29s)

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