Table 1 Naturally occurring major CRISPR-Cas enzymes
| Â | Size | PAM sequence | Size of sgRNA guiding sequence | Cutting site | Reference |
|---|---|---|---|---|---|
spCas9 | 1368 | NGG | 20 bp | ~ 3 bp 5′ of PAM | Jinek et al.42 |
Gasiunas et al.43 | |||||
FnCas9 | 1629 | NGG | 20 bp | ~ 3 pb 5′ of PAM | Hirano et al.60 |
SaCas9 | 1053 | NNGR RT | 21 bp | ~ 3 pb 5′ of PAM | Mojica et al.57 |
NmCas9 | 1082 | NNNNG ATT | 24 bp | ~ 3 bp 5′ of PAM | Hou et al.53 |
St1Cas9 | 1121 | NNAGA AW | 20 bp | ~ 3 bp 5′ of PAM | Gasiunas et al.43 |
Cong et al.45 | |||||
St3Cas9 | 1409 | NGGNG | 20 bp | ~ 3 bp 5′ of PAM | Gasiunas et al.43 |
Cong et al.45 | |||||
CjCas9 | 984 | NNNNACAC | 22 bp | ~ 3 bp 5′ of PAM | Kim et al.56 |
AsCPf1 | 1307 | TTTV | 24 bp | 19/24 bp 3′ of PAM | Yamano et al.50 |
Kim et al. 2016 | |||||
LbCpf1 | 1228 | TTTV | 24 bp | 19/24 bp 3′ of PAM | Yamano et al.50 |
Kim et al. 2016 | |||||
Cas13 | Multiple orthologs | RNA targeting | 28 bp |  | Abudayyeh et al. 2017 |
