Fig. 4

PHP is not expressed in hiw mutants or wnd-OE following acute postsynaptic GluR perturbation. a Schematic and representative EPSP and mEPSP traces of indicated genotypes before (baseline) and following PhTx application. Quantification of mEPSP (b) and EPSP (c) amplitude in the indicated genotypes and conditions. Note that while PhTx application causes a reduction in mEPSP amplitudes in all genotypes, only hiw mutants and wnd-OE fail to maintain baseline EPSP amplitudes. d Quantification of mEPSP and quantal content values normalized to the same genotype in the absence of PhTx (baseline). No increase in presynaptic neurotransmitter release (quantal content) is observed in hiw+PhTx or wnd-OE+PhTx, demonstrating that PHP fails to be acutely expressed in hiw mutants, but is restored in hiw;wnd double mutants. Error bars indicate ± SEM. For statistical significance, an unpaired t-test was performed between respective genotypes with and without PhTx. **p 0.01; ****p 0.0001; NS not significant, p > 0.05