Fig. 2

Identification of intrinsic molecular subtypes of colorectal liver metastases. a Consensus molecular subtypes (CMS) of primary colorectal cancers obtained from the Colorectal Cancer Subtyping Consortium (CRCSC) or calculated in primary colorectal cancers of The Cancer Genome Atlas (TCGA). CMS subtypes were also determined in colorectal liver metastases from patients undergoing partial hepatectomy of resectable liver metastases (UC, NS, MSK1, and MSK2 cohorts) or biopsy of unresectable liver metastases (MSK3, Italian and French cohorts). Cohorts contain independent clinical and molecular datasets (see Supplementary Information). b Consensus clustering based on similarity network fusion (SNF) subtyping of colorectal liver metastases. Subtype 1 = S1, Subtype 2 = S2, Subtype 3 = S3. c Kaplan–Meier curves of overall survival by molecular subtype. P-value was determined using a log-rank test. d Metastatic recurrence patterns by molecular subtype. Asterisks denote statistical significance based on Fisher’s exact test for each subtype versus the two other subtypes. e Differentially expressed mRNAs (left) and miRNAs (right) between the three molecular subtypes (see Supplementary Data 2 and 3). Subtype 1 = red, Subtype 2 = green, Subtype 3 = blue