Fig. 2
HN2 treatment induces Saw1-GFP foci. a HN2-induced Saw1-GFP and saw1db--GFP (DNA binding-deficient saw1 variant) foci formation. Saw1-GFP but not saw1DB-GFP make nuclear foci upon HN2 treatment (1 h, p value < 0.0078, 2 h, p value < 0.0048 using student t-test). b (Top) representative images of cells bearing HN2-induced Saw1-GFP and Rad52-mRFP. (Bottom) images of MMS-induced Saw1-GFP and Rad52-mRFP. DIC images are shown for cell shape. c Percentage of cells bearing Saw1-GFP or Rad52-mRFP upon indicated drug treatments. Shown are the average + s.d. of three independent experiments. At least 100 cells were counted per experiment. Saw1-GFP and Rad52-GFP co-localize upon MMS but not on HN2 treatment (p value < 0.012 using student t-test)
