Fig. 5
From: Footprints of parasitism in the genome of the parasitic flowering plant Cuscuta campestris
Horizontal gene transfer (HGT) in C. campestris. a Depiction of the phylogenetic origin of 36 HGT events and their functional category assignment. Phylogenetic orders from which HGT originated are color-coded according to their evolutionary distance to Cuscuta using the median values in million years (MYA) for the split as predicted by Timetree (http://timetree.org) and supported by a species tree calculated using Astral-II (Supplementary Fig. 5). White numbers refer to the number of genes per donor order, black numbers to the right indicate the number of genes in the recipient, C. campestris. Cake diagrams represent functional assignment of donor genes in MapMan bins17. b Phylogenetic placement of HGT candidate protein Cc027215, a G-type lectin S-receptor-like serine/threonine-protein kinase, Callus Expression of RBCS 101 (CES101). The amino acid sequence of Cc027215 (highlighted in yellow) is nested deeply within a cluster of orthologous proteins from the hypothetical donor order Fabales (in red), while all orthologs from Solanales species (in gray), that are much more closely related to C. campestris, are located on the second branch. The schematic sequence comparison shows identities relative to Cc027215 in black boxes, discrepancies in gray boxes and gaps in the alignment as black lines. Bootstrap values after 1000 replicates were 86% or higher for all nodes. c Nucleotide sequence comparison of a 36 kb region of chromosome 3 from Daucus carota (top) and a region of C. campestris scaffold123. Coding sequences are shown in dark green and introns as well as up- and down-stream untranslated flanking regions are shown in light green. Areas with very high similarity, indicating conserved remnants of a previous horizontal DNA transfer between both species are indicated by gray fields. Within the depicted area, a gene duplication (Cc021601 and Cc021599 both mapping with all three exons and two introns to DCAR_012700), a larger sequence insertion between Cc021599 and Cc021598, as well as sequence inversions can be seen
