Fig. 6
From: Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS
SFPQ nuclear clearance is a molecular hallmark of genetic and sporadic ALS. a Analysis of the subcellular localisation of SFPQ in MNs in the ventral spinal cord of wild-type, VCPA232E and SOD1G93A mice. MN cytoplasm was identified by ChAT staining, nuclei were counterstained with DAPI. Data shown is nuclear/cytoplasmic (N/C) ratio (mean ± s.d.) per cell from three wild-type, 4 SOD1G93A and 3 VCPA232E mice. Scale bar: 20 μm. P-values from one-sided Welch’s t-test. Cells from individual animals in each condition were pooled together after excluding individual mice effect by comparing full linear (disease and individual factor) with reduced linear (disease only) models using the Akaike Information Criterion. b Analysis of the subcellular localisation of SFPQ in MNs in the ventral spinal cord of healthy controls and patients with sporadic ALS (sALS). MN cytoplasm was identified by ChAT staining, nuclei were counterstained with DAPI. Only MNs with a visible nucleus were considered for the analysis. Scale bar 50 μm. Data shown is N/C ratio (mean ± s.d.) per cell from three cases per group
