Fig. 1

Expression of Dlx1 in the developing ARC. a, b In situ hybridization (ISH) for Dlx1 and Dlx2 (a) and ISH for GHRH combined with immunohistochemistry (IHC) for Dlx1 (b). A total of 88.5 ± 5% of GHRH⁺ cells were Dlx1⁺ cells in IHC (b). c, d IHC analyses of Dlx1 expression in Pomc-eGfp (c) and Npy-hrGfp (d) embryos using our home-made antibodies against Dlx1. In Pomc-eGfp, none of GFP⁺ cells was Dlx1⁺ in IHC (c). In E14.5 Npy-hrGfp, 15 ± 1% of GFP⁺ cells were Dlx1⁺ in IHC, while 93 ± 1% of Dlx1⁺ cells did not express GFP (d). In E17.5 Npy-hrGfp, 12 ± 2% of GFP⁺ cells were Dlx1⁺ in IHC, while 90 ± 3% of Dlx1⁺ cells did not express GFP (d). Quantification was performed with multiple embryos and at least three sections from each embryo, and representative images for only one side of the ARC are as shown. Scale bars, 100 μm