Fig. 1
Effect of EP overexpression on oligomer Aβ-induced hippocampal synaptic deficit. Hippocampal slices were derived from 3-month-old non-Tg and Tg Sh3gl2 mice. Slices were perfused with 50, 100, and 200 nM Aβ for 1 h. a Fifty nanomolar of oligomer Aβ perfusion over non-Tg slices does not significantly reduce LTP level. Perfusion of non-Tg hippocampal slices with higher Aβ concentration (100 nM, 200 nM) impairs hippocampal LTP expression. Error bars represent s.e.m., n = 7–10 per group; #p < 0.05 (one-way ANOVA). b Fifty nanomolar of oligomer Aβ perfusion over Tg Sh3gl2 slices significantly reduce the LTP level. Perfusion with higher Aβ concentration (100 nM, 200 nM) saturated the deleterious effect of Aβ on hippocampal LTP impairment. Line indicates Aβ exposure period. Error bars represent s.e.m., n = 7–10 per group; #p < 0.05 (one-way ANOVA). c Upper panels of a and b show representative traces of fEPSP in slices with the indicated treatment before θ-burst stimulation (black line) and after 1 h (gray line). Summarized LTP levels (average of the fEPSPs slope of the last 10 min recordings) in the indicated groups. Data are shown as mean ± s.e.m., n = 7–10 per group; #p < 0.05 (Student’s t test)
