Fig. 5
From: Protein polarization driven by nucleoid exclusion of DnaK(HSP70)–substrate complexes
FRAP analysis reveals Ci and DnaK-RFP exchanges between LDCs at opposite poles. a Scheme of the analytical procedure. Empty circle: photo-bleached area. Dotted area: regions subjected to FRAP analysis, corresponding to: the FRAP area (P, blue), the opposite pole (I, red), the mid- (M, gray) or whole bacterial body (W, black). b, d–f FRAP analysis. Left panels: representative time-lapse images of bacteria subjected to FRAP analysis. The time after bleach is indicated in seconds. Scale bar = 5 μm. Right: representative fluorescence recovery kinetics of area corresponding to FRAP area (P, blue), opposite pole (I, red) and mid-bacterial body (M, gray) analysis. The values are expressed as relative percent of the residual average intensity in whole bacterial body (W, black) after photobleaching. c Average half-recovery time calculated from single exponential fits for at least 30 measurements in three independent experiments. Asterisks indicate statistical significance determined by Dunn’s test: p < 3.8 × 10-12. b, d MC4100/pDnaK-RFP grown at 30 °C (b) or 37 °C (d). e MC4100/pCi. f dnaKJ/pCi. The red circles correspond to analysis in a Ci patch adjacent to the photobleached area
