Fig. 7
From: Protein polarization driven by nucleoid exclusion of DnaK(HSP70)–substrate complexes
Ci folding is assisted by DnaKJE. a Native gel analysis of DnaKJ-Ci complexes. Indicated purified proteins were mixed and heated for 10 min at 45 °C prior to native gel analysis and western-blotting with antibody directed against the indicated protein. O with a slash indicates Ci alone. Left: anti-IpaC western blot with short (top) and long (bottom) exposure time. Arrow: Ci-induced shift; solid line: DnaK-induced shift; dotted line: native major band. b, c Refolding assay of acid-denatured Ci in the presence of the indicated chaperone. Data points represent the mean value from three independent experiments. For each value, the standard deviation is less than 10%. Sample mix: K: DnaK; J: DnaJ; E: GrpE. Ci, DnaK, DnaJ, and GrpE were incubated at the final concentration of 3, 3, 1, and 0.6 μM, respectively. b The dotted line represents the maximal slope used to determine the initial folding rate
