Fig. 8
From: Protein polarization driven by nucleoid exclusion of DnaK(HSP70)–substrate complexes
GrpE controls LDC dynamics. Following induction of Ci expression (IPTG), bacterial strains were incubated in the presence of arabinose ( + Ara) for 2 h. CTRL: MC4100/pSUCi/pBAD22; + GrpE: MC4100/pSUCi/pBAD22-GrpE. a Bacterial lysates were analyzed by western-blotting with the indicated antibody. b, c Samples were fixed and processed for fluorescence microscopy analysis. b Representative micrographs. Red: DAPI staining; Green: Ci fluorescence. Scale bar = 5 µm. c Percent (mean ± SEM) of bacteria ( > 150 bacteria, N = 2) with fluorescent signal (Fluo), and bacteria with polar Ci signal (Pol). Statistical significance was determined by ANOVA with Tukey’s post hoc analysis (**, P ≤ 0.01; ns, not significant P > 0.05)
