Fig. 5
From: Cellular conditions of weakly chelated magnesium ions strongly promote RNA stability and catalysis
Coordinated water molecules are critical for RNA stabilization. a Melting curves of drz-spur-3 ribozyme in the background of metabolites. 3 mM Mg2+total (orange) and 28 mM of glutamate (red), malate (green), citrate (cyan), or EDTA (black) conditions were tested. As a control, 1.1 mM Mg2+total (blue) condition was performed. The estimated concentrations of Mg2+free are provided. KD’s are given in the main text. b Chemical structure of glutamate and magnesium. Glutamate interacts with magnesium through N, O-chelation. c Chemical structure of EDTA and magnesium. EDTA interacts with magnesium through O, O, O, O, N, N-chelation
