Fig. 8 | Nature Communications

Fig. 8

From: Plasma membrane LAT activation precedes vesicular recruitment defining two phases of early T-cell activation

Fig. 8

Two-phase model for LAT activation. A model for LAT microcluster formation and maintenance using the two cellular pools of LAT is shown. a The left panel shows that at early time points, LAT vesicles decorated with VAMP7 are several microns away from the immune synapse, while plasma membrane-resident LAT can move laterally to be recruited to microclusters. The middle and right panels show that microtubule polarization toward the immunological synapse causes the reorientation of various vesicular compartments to the subsynaptic zone. b, c Zoomed-in regions of early and late stages of activation, respectively. b At early time points, plasma membrane LAT is recruited to microclusters and activated ZAP-70 phosphorylates LAT at the PM. c At later time points, LAT/VAMP7 vesicles are recruited to the immune synapse, move in a directed manner between microclusters, and interact dynamically with microclusters

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