Fig. 4
From: CREB controls cortical circuit plasticity and functional recovery after stroke
Lentiviral CREB expression induces greater multi-joint movements after stroke. a Immunohistochemical staining of pyramidal cells (CAMKIIα promoter drives expression in pyramidal neurons) in peri-infarct M1 in mice transfected with Lentiviral ChR2-mCherry (left) and lentiviral CREB-eGFP (middle). Right: Co-localization of ChR2 and CREB lentiviruses 4 weeks after stroke, showing nearly complete co-transfection with these two viruses. Scale bar = 50 μm. b Whole cell recording shows a pyramidal cell (cortex) transfected with lenti-ChR2/mCherry. The cell displayed action potentials (AP) in cell attached mode with blue light (4 mW) and tracked higher frequency light stimulation (10 Hz). At −70 mV, blue light (4 mW, 0.5 ms pulse) triggers an inward current as expected. c Setup of head-fixed mouse allowed to run freely on a floating Styrofoam ball to detect multi-joint movements during brief pulses of blue light stimulation (pulse length 5 ms, pulse frequency 20 Hz). d Graph shows the percentage of multi-joint complex movements during light stimulation. Stroke + ChR2-CREB produced a significant increase in multi-joint movements compared with stroke + ChR2-alone 4 weeks after stroke
