Fig. 2
From: High-throughput screening of prostate cancer risk loci by single nucleotide polymorphisms sequencing
Overall study design. SNPs-seq workflow (left panel): 374 SNPs were selected from analyzing GWAS, ChIP-seq, and eQTL data. SNP-containing oligos were synthesized, followed by annealing the positive and negative strands to make ds-oligos. By mixing ds-oligos with nuclear extract, the protein-bound ds-oligos were separated and used for library preparation and allele-specific sequencing analysis. STARR-seq workflow (right panel): Significant SNPs selected from SNPs-seq were PCR-amplified, pooled and inserted into STARR-seq vector. After co-transfecting LNCaP cells, the mRNAs from transfected cells were isolated and used to make STARR-seq library for allele-specific sequencing analysis
