Fig. 5 | Nature Communications

Fig. 5

From: Increased glutarate production by blocking the glutaryl-CoA dehydrogenation pathway and a catabolic pathway involving l-2-hydroxyglutarate

Fig. 5

Production of glutarate in mutant of glutarate hydroxylation pathway and glutaryl-CoA dehydrogenation pathway. a The metabolic engineering strategies for the production of glutarate from l-lysine in P. putida KT2440. In this work, gdh, csiD and alr were inactivated individually or in combination for improvement of glutarate yield. alr, alanine racemase; davBA, l-lysine monooxygenase and 5-aminovaleramide amidohydrolase; davT, 5-aminovalerate aminotransferase; davD, glutaric semialdehyde dehydrogenase; 2-KG, 2-ketoglutarate. Growth (b) and the production of glutarate (c) by wild-type P. putida KT2440 and mutants cultured in l-lysine were compared. The consumption of l-lysine (red lines) and the yield of glutarate (blue lines) were shown. Growth (d) and the production of glutarate (e) by wild-type P. putida KT2440 and mutants cultured in the medium with l-lysine and glucose were compared. The consumption of l-lysine (red lines), the consumption of glucose (purple lines) and the yield of glutarate (blue lines) were shown. f Comparison of the consumption of l-lysine, the yield of glutarate and the conversion ratio using wild-type P. putida KT2440 and mutants cultured in the medium with l-lysine and glucose. Data shown are mean ± s.d. (n = 3 independent experiments)

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