Fig. 7 | Nature Communications

Fig. 7

From: Increased glutarate production by blocking the glutaryl-CoA dehydrogenation pathway and a catabolic pathway involving l-2-hydroxyglutarate

Fig. 7

Metabolic pathways of glutarate in P. putida KT2440. a Analyses of the end products of the glutaryl-CoA dehydrogenation pathway and the glutarate hydroxylation pathway. b Comparison of the growth of P. putida KT2440 and its derivatives on glutarate. The biomass of P. putida KT2440 and its derivatives were estimated as the optical density at 600 nm after 12 h cultivation. Data shown are mean ± s.d. (n = 3 independent experiments). c Schematic representation of pathways for glutarate metabolism in P. putida KT2440. Blue-colored arrows indicate the glutaryl-CoA dehydrogenation pathway and green-colored arrows indicate the glutarate hydroxylation pathway. The acetyl-CoA generated from the glutaryl-CoA dehydrogenation pathway can be condensed to C4 compound via the glyoxylate cycle (red-colored arrows). AceA, isocitrate lyase; GlcB, malate synthase. GOX, glyoxylate; CIT, citrate; ICT, isocitrate; SUC, succinate; FUM, fumarate; MAL, malate; OAA, oxaloacetate; 2-KG, 2-ketoglutarate; l-2-HG, l-2-hydroxyglutarate

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