Fig. 5
From: Induction of anergic or regulatory tumor-specific CD4+ T cells in the tumor-draining lymph node
CpG administration at the tumor site does not restore an effector CD4+ T cell response. a Mice bearing advanced tumors received 4 μg of CpG twice, at 5-day interval, 2 days before Marilyn cell transfer. b Proliferation profile of Marilyn cells in the TdLN of tumor-bearing mice receiving or not CpG. c Frequency of ≥5 times divided Marilyn cells. d Representative plots showing the frequency of Marilyn cells in the lung and e quantification (number). f Representative plots showing the frequency of FOXP3+ Marilyn cells in the TdLN and g quantification (number). h Representative plots of FR4 and CD73 expression on CD44hiFOXP3− Marilyn cells, and i quantification (number of anergic or Teff-mem cells). ns: non-significant, *p<0.05, **p<0.01 Mann–Whitney U test. One representative experiment out of two is depicted
