Fig. 6
From: Induction of anergic or regulatory tumor-specific CD4+ T cells in the tumor-draining lymph node
Concomitant administration of DBY and CpG at the tumor site reinforces tumor-mediated immunosuppression. a Tumor-bearing mice were i.t. injected twice with DBY peptide (200 ng) and CpG (4 μg) at 5-day interval followed 2 days after by CFSE-labeled naive Marilyn cell transfer. b Pattern of proliferation of Marilyn cells in the TdLN and c quantification (frequency of ≥5 divisions). d Representative plots showing the frequency of Marilyn cells in the lung and e quantification (number). f FOXP3 expression by Marilyn cells and g quantification (number). h Representative FR4 and CD73 expression on CD44hiFOXP3− Marilyn cells and i quantification (number of anergic or Teff-mem cells). ns: non-significant, *p<0.05, **p<0.01 Mann–Whitney U test
