Fig. 9
From: Induction of anergic or regulatory tumor-specific CD4+ T cells in the tumor-draining lymph node
Priming of tumor Ag-specific naive CD4+ T cells at distant site from the tumor restores their effector functions. a Tumor-bearing mice having received CFSE-labeled naive Marilyn cells were immunized into the f.p. with DBY peptide (200 ng) and CpG (40 μg) emulsified in IFA or left untreated. Analysis was performed 7 days later. b Pattern of proliferation of Marilyn cells in the TdLN, lymph nodes draining the Ag injection site (IdLN = popliteal + Inguinal-LNs) and ndLN (non-draining LN = Inguinal) and c quantification (frequency of ≥5 divisions). d Representative plots showing accumulation of Marilyn cells in the lung of immunized tumor-bearing mice and e quantification (number). f Representative expression of CD73 and FR4 by CD44hiFOXP3− Marilyn cells and g quantification (number of anergic or Teff-mem cells) h, i Frequency of IFN-γ-producing Marilyn cells in the lung. One representative experiment out of two is depicted. ns: non-significant, **p<0.01, ***p<0.001 Mann–Whitney U test
