Fig. 4
From: Spatial self-organization resolves conflicts between individuality and collective migration
Phenotypes spontaneously order themselves along the traveling band according to tumble bias. a The distribution of tumble bias in the population can be controlled by manipulating the level of expression of the phosphatase CheZ, which deactivates the chemotactic response regulator CheY (Supplementary Fig. 9a). b Time-lapse coordinates (colored dots) of an equal mixture of low (red in a) and high (cyan in a) TB cells traveling in 200 µM aspartate M9 glycerol buffer (see Methods). Scale bar, 0.6 mm. c Corresponding density profiles (colors) together with total cell density (black). (Line: mean over n = 34 time points measured at 40 s intervals for one experiment; shading: SD; five replicates are in Supplementary Fig. 9b). d Same as in c, but for the magenta and green populations in a. Two replicates are in Supplementary Fig. 9c. e Peak positions as a function of time for the experiment in b. f The distance between the fluorescence intensity peaks of the two populations traveling together in a single band increases with the difference between the mean TB of the two populations. For each independent experiment, two populations labeled with different fluorescent proteins (mRFP1 or YFP) were induced using different aTc levels to obtain distributions with different mean tumble biases. Dots: average over n = 4 experiments; error bars are SD
